What the technology does
Polymerase with enhanced ability to process bisulphite-treated DNA and hydrophobic base analogues.
Advantage
Compared to the standard enzyme of choice (Taq), polymerase 5D4 has enhanced DNA synthesis efficiency on bisulphite-treated DNA and enhanced bypass of dhU6S adducts in bisulphite-treated non-desulphonated DNA. It also has improved sensitivity in PCR amplification of bisulphite-treated DNA or uracil-containing DNA templates in general.
Main application field
Polymerase 5D4 has utility in bi-sulphite sequencing and in processing of uracil-containing DNA templates. It was originally selected for its ability to process hydrophobic base analogues, so would have wider application in this field
Usage
R&D tool
Status
Technology ready for evaluation and licensing
Intellectual Property
Granted patents include EP2145956 and US9096835.
Offering
Non-exclusive licence