What the technology does
Polymerase with enhanced ability to process bisulphite-treated DNA and hydrophobic base analogues.
Compared to the standard enzyme of choice (Taq), polymerase 5D4 has enhanced DNA synthesis efficiency on bisulphite-treated DNA and enhanced bypass of dhU6S adducts in bisulphite-treated non-desulphonated DNA. It also has improved sensitivity in PCR amplification of bisulphite-treated DNA or uracil-containing DNA templates in general.
Main application field
Polymerase 5D4 has utility in bi-sulphite sequencing and in processing of uracil-containing DNA templates. It was originally selected for its ability to process hydrophobic base analogues, so would have wider application in this field
Technology ready for evaluation and licensing
Granted patents include EP2145956 and US9096835.